Journal: Frontiers in Immunology

Article Title: Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

doi: 10.3389/fimmu.2018.02178

Figure Lengend Snippet: Role of Toll-like receptor (TLR)-2 and TLR4 in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).

Article Snippet: Unlabeled anti-porcine TLR2 and TLR4-rabbit IgG antibodies (Biolegend, San Diego, CA) were used to block TLRs expression in PIE cells as described previously ( ).

Techniques: Activity Assay, Incubation, Expressing, Control, Blocking Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Identification, cloning, and recombinant expression of procalin, a major triatomine allergen.

doi: 10.4049/jimmunol.167.5.2694

Figure Lengend Snippet: FIGURE 5. Immunolocalization of 19-kDa salivary allergen of T. pro- tracta. Salivary glands or whole bugs were fixed, embedded, and sectioned as described in Materials and Methods. A, Control Ab; B, anti-procalin rabbit antisera (1:1000) as primary Ab. Alkaline phosphatase-linked sec- ondary Ab (goat anti-rabbit) reaction localizes allergen to salivary gland epithelial cells and contents of salivary gland lumen (original magnifica- tion, 200).

Article Snippet: Sections (2.5 m) were cut and incubated overnight at 4°C with a 1/1000 dilution of hyperimmune rabbit antiserum reactive with the recombinant protein, which were washed and incubated with a 1/100 dilution of alkaline phosphatase-labeled goat anti-rabbit Ab by using the Vectastain ABC (avidin-biotin complex)-alkaline phosphatase kit (Vector Laboratories, Burlingame CA) (17, 18).

Techniques: Control